Lipoprotein, High Density (HDL) and Lipoprotein, Low Density (LDL)/Lipoprotein, Very Low Density (VLDL) BioAssay(TM) Kit, Colorimetric

Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. \n\nSimple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using cholesterol esterase/cholesterol dehydrogenase reagent. In this reaction, NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.\n\nApplications:\nDirect Assays: HDL and LDL/VLDL cholesterol in serum samples from any species.\nPharmacology: evaluation of drugs on cholesterol metabolism.\n\nKey Features:\nSensitive and accurate. Requires only 20ul serum sample.\nDetection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay.\nConvenient. Room temperature assay. No 37 degrees C heater is needed.\n\nKit Contents: \n100 assays in 96-well plates\nPBS: 1.5ml Precipitation Reagent: 1.5ml\nAssay Buffer: 20ml Enzyme Mix: 120uL\nNAD Solution: 2ml Standard: 1ml 300mg/dL cholesterol\n\nStorage conditions. Store PBS and Precipitation Reagent at room temperature and the rest reagents at -20 degrees C. Shelf life: 6 months after receipt.\nPrecautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.\n\nProcedures:\nImportant: bring all reagents except enzyme mix to room temperature prior to assay. Non-hemolyzed serum samples should be used. The following procedure is designed for duplicate determinations.\n1. Sample Preparation. Transfer 20ul serum into a 1.5-mL centrifuge tube, add 20ul Precipitation Reagent. Vortex to mix and centrifuge 5 min at 9,500 x g (e.g. 9,500 rpm in an Eppendorf 5415C tabletop centrifuge). Carefully transfer 24ul supernatant into a clean tube, add 96ul Assay Buffer. Label this tube

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

L2600-57B

Size

1Kit

References

1. Tam, J. et al. (2010). Peripheral CB1 cannabinoid receptor blockade improves cardiometabolic risk in mouse models of obesity. J Clin Invest. 120(8): 2953-66.\n2. Uddin, M. J. et al. (2011). Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genetics 12: 62.\n3. Shon, S-M. et al. (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73.