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Lipoprotein, High Density (HDL) and Lipoprotein, Low Density (LDL)/Lipoprotein, Very Low Density (VLDL) BioAssay(TM) Kit

Cat no: L2600-56

Lipoprotein, High Density (HDL) and Lipoprotein, Low Density (LDL)/Lipoprotein, Very Low Density (VLDL) BioAssay(TM) Kit

Regulation of HDL (high-density-lipoprotein)-cholesterol and LDL (low-density-lipoprotein)-cholesterol plays a central role in various disease developments. It is well known that low levels of HDL and high level of LDL are associated with an increased risk of cardiovascular events. The HDL and LDL/VLDL Cholesterol Quantification Kit provides a simple\nquantification method of HDL and LDL/VLDL after a convenient separation of HDL from LDL\nand VLDL (very low-density lipoprotein) in serum samples. In the assay, cholesterol oxidase\nspecifically recognizes free cholesterol and produces products which react with probe to\ngenerate color (lambda = 570nm) and fluorescence (Ex/Em = 538/587nm). Cholesterol esterase hydrolizes cholesteryl ester into free cholesterol, therefore, cholesterol ester and free\ncholesterol can be detected separately in the presence and absence of cholesterol esterase\nin the reactions.\n\nKit Components:\nCholesterol Assay Buffer 1x25ml\n2X LDL/VLDL Precipitation Buffer 1x10ml\nCholesterol Probe (in DMSO, anhydrious) 1x200ul\nEnzyme Mix (Lyophilized) 1x1vial\nCholesterol Esterase (Lyophilized) 1x1vial\nCholesterol Standard (2ug/ul) 1x100ul.\n\nStorage and Stability:\nStore the kit at -20 degrees C, protect from the light. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L2600-56

Size

100Tests

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