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LOXL2, Recombinant, Human (Lysyl Oxidase-like Protein 2, Lysyl Oxidase Related Protein 2, LOR2, Lysyl Oxidase Homolog 2, Lysyl Oxidase Related Protein WS9-14)

Cat no: L2623-15

LOXL2, Recombinant, Human (Lysyl Oxidase-like Protein 2, Lysyl Oxidase Related Protein 2, LOR2, Lysyl Oxidase Homolog 2, Lysyl Oxidase Related Protein WS9-14)

Source:\nRecombinant corresponding to aa500-end of human LOXL2 expressed in a baculovirus-infected Sf9 cell expression system (NM_002318).\n\nActivity: \nEnzyme activity is assayed by an AmplexRed/Peroxidase coupling method using 1,5-diaminopentane as a substrate. Purified LOXL-2 (1-2 ng/ul) is incubated with 3mM 1,5-diaminopentane, 0.4mM AmplexRed and 0.2unit/ul peroxidase in 100ml of the assay buffer (1.2M Urea, 50mM borate, pH 8.8) for 30 min at 30C. Fluorescence intensity is measured every 7 minute at 530ex/590em. \n\nApplications: \nSuitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing. Store at -70 degrees C. Aliquots are stable for at least 6 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L2623-15

Size

10ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 3mM DTT, 20% glycerol.

Purity

Purified ~65%

References

1. Peng L. et al., Carcinogenesis 30(10), 1660-1669 (2009). 2. Fujimoto E. and Tajima S. J. Dermatol. Sci. 55(2), 91-98 (2009).

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