LOXL3, Recombinant, Human (Lysyl Oxidase Homolog 3, Lysyl Oxidase-like Protein 3, LOXL)

Lysyl oxidase-like protein 3 (LOXL3) is a secreted copper amine oxidase. The N-terminal region of LOXL3 contains four scavenger receptor cysteine- rich (SRCR) domains. The C-terminal region consists of a catalytic domain similar to other lysyl oxidases and a cytokine receptor-like domain. The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone cofactor. LOXL3 is expressed primarily in epidermis, kidney, aorta, and chondrocytes. The enzyme is expressed at low levels in most other tissues. Like lysyl oxidase, LOXL3 oxidizes the lysine epsilon-amino groups of a number of collagen substrates, and is also active against other primary amines. However, LOXL3 does not require proteolytic processing to be activated.\n\nSource: \nChinese Hamster Ovary cell line, CHO-derived Met1-Ile753, with a C-terminal 10-His tag.\n\nSDS-PAGE:\n90-100kD, reducing conditions\n\nActivity:\nMeasured by its ability to produce to produce hydrogen peroxide during the oxidation of benzylamine.\n\nSpecific Activity:\n(same/more than)1.5pmoles/min/ug\n\nEndotoxin Level: (same/less than)1EU/1ug (LAL)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L2623-16

Size

10ug

Form

Supplied as a liquid in MES, sodium chloride.

Purity

~90% (SDS-PAGE, reducing conditions)

References

1. Csiszar, K. (2001) Prog. Nucl. Acid Res. Mol. Biol. 70:1. 2. Maki, J.M. and K.I. Kivirikko (2001) Biochem J. 355:381. 3. Molnar, J. et al. (2003) Biochim. Biophys. Acta 1647:220. 4. Lee, J. and Y. Kim (2006) J. Biol. Chem. 281:37282.