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LSD1 (Lysine-Specific Histone Demethylase, Amine Oxidase Flavin-containing Domain Protein 2, AOF2)

Cat no: L4595-50

LSD1 (Lysine-Specific Histone Demethylase, Amine Oxidase Flavin-containing Domain Protein 2, AOF2)

Description:\nHistone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine- specific demethylation of histone proteins via an FAD- dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in \nheterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3- K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression. \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug

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SPECIFICATIONS

Catalog Number

L4595-50

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Shi Y, et al., (2004) Cell 119:941-53. 2. Kouzarides T. (2002) Curr. Opin. Genet. Dev. 12:198-209. 3. Shi YJ, et al., (2005) Mol. Cell 19:857-64. 4. Nakamura T, et al. (2002) Mol. Cell 10:1119-28. 5. Lee MG, et al. (2005) Nature 437:432-5. 6. Hakimi MA, et al. (2002) Proc. Natl. Acad. Sci. USA 99:7420-5.

Additional Info

Recognizes human LDS1. Species Crossreactivity: mouse and rat

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