LSP1 (Lymphocyte-specific Protein 1, 47kD Actin-binding Protein, 52kD Phosphoprotein, pp52, Lymphocyte-specific Antigen WP34, WP34)

Leukocyte-specific protein (LSP; also known as Lymphocyte-specific protein1, SP34 and pp52) is a 52kd filamentous-actin-binding intracellular phosphoprotein. LSP is localized to the internal surface of the plasma membrane, cytoplasm, and actin filaments. LSP expressed in various eukaryotic cell lines such as the melanoma cell line A7 but not in other hematopoietic lineages. Studies showed that LSP play a key role in anti-IgM-induced apoptosis of the B-cell lymphoma line as well as cytoskeleton-driven regulation. LSP contains a high-affinity F-actin binding site, which recently is identified in the basic C-terminal half.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:16,000\nWestern Blot: 0.05-0.2ug/ml, observed in human lymph node, tonsil and peripheral blood mononucleocytes lysates on ~50kD bands\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L2030-30C

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Bov, Can

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Gringhuis SI, den Dunnen J, Litjens M, van der Vlist M, Geijtenbeek TB, Carbohydrate-specific signaling through the DC-SIGN signalosome tailors immunity to Mycobacterium tuberculosis, HIV-1 and Helicobacter pylori. Nature immunology 2009 Oct 10(10):1081-8.

Additional Info

Recognizes human LSP1. Species Crossreactivity: Mouse and rat. Species sequence homology: Bovine and canine.