$LY294002 (PI 3 Kinase Inhibitor, 2-(4-Morpholinyl)-8-phenyl-4H-1-\nbenzopyran-4-one)$

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Cat no: L7751

LY294002 (PI 3 Kinase Inhibitor, 2-(4-Morpholinyl)-8-phenyl-4H-1-\nbenzopyran-4-one)

LY294002 is a potent and specific cell-permeable inhibitor of phosphatidylinositol 3-kinase (PI3-kinase). LY294002 at a concentration of 50uM completely abolishes the PI3-K activity of intact neutrophils stimulated with fMet-Leu-Phe, without apparent cell toxicity. While concentration of 50uM is observed to be efficacious for inhibiting PI3-K activity in stimulated neutrophils, this concentration produces no significant inhibition of other kinases including PKC, PKA, MAP kinase, S6 kinase, EGF receptor tyrosine kinase, Src, PI4-kinase, diacylglycerol kinase, or rabbit kidney ATPase. This compound is a useful tool for identifying cellular event that are regulated by the PI3-kinase/Akt axis, and is observed to induce apoptosis in many cell types by blocking the PI3-kinase/Akt anti-apoptotic pathway.\n\nLY294002 (PI3 Kinase Inhibitor) was shown to act in vivo as a highly selective inhibitor of phosphatidylinositol 3 (PI3) kinase. When used at a concentration of 50uM, it specifically abolished PI3 kinase activity (IC50=0.43ug/ml; 1.40uM) but did not inhibit other lipid and protein kinases such as PI4 kinase, PKC, MAP kinase or c-Src (1). LY294002 has been shown to block PI3 kinase-dependent Akt phosphorylation and kinase activity.\n\nApplications: Sutiable for use in vitro and in vivo.\n\nRecommended Dilutions:\nCultured cells: Recommend treating the cells with LY294002 for one hour prior to, and for the duration of, the stimulation.\nOptimal dilutions to be determined by researher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Light sensitive. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L7751

Size

500ug

Form

Supplied as a liquid in DMSO.

References

1. Guo, M., et al., (2000) Biochem. Pharmacol. 60: 635-642. 2. Martin, M.C., et al., (2001) J. Biol. Chem. 276: 45041-45050. 3. Panka, D.J., et al., (2001) J. Biol. Chem. 276: 6,893-6,896. 4. Schwartzbauer, G. and Robbins, J., (2001) J. Biol. Chem. 276: 35786-35793. 5. Vlahos, C., et al., (1994) J. Biol. Chem. 269: 5241-5248.