LY294002 (PI 3 Kinase Inhibitor, 2-(4-Morpholinyl)-8-phenyl-4H-1- benzopyran-4-one)

LY294002 is a potent and specific cell-permeable inhibitor of phosphatidylinositol 3-kinase (PI3-kinase). LY294002 at a concentration of 50uM completely abolishes the PI3-K activity of intact neutrophils stimulated with fMet-Leu-Phe, without apparent cell toxicity. While concentration of 50uM is observed to be efficacious for inhibiting PI3-K activity in stimulated neutrophils, this concentration produces no significant inhibition of other kinases including PKC, PKA, MAP kinase, S6 kinase, EGF receptor tyrosine kinase, Src, PI4-kinase, diacylglycerol kinase, or rabbit kidney ATPase. This compound is a useful tool for identifying cellular event that are regulated by the PI3-kinase/Akt axis, and is observed to induce apoptosis in many cell types by blocking the PI3-kinase/Akt anti-apoptotic pathway. LY294002 (PI3 Kinase Inhibitor) was shown to act in vivo as a highly selective inhibitor of phosphatidylinositol 3 (PI3) kinase. When used at a concentration of 50uM, it specifically abolished PI3 kinase activity (IC50=0.43ug/ml; 1.40uM) but did not inhibit other lipid and protein kinases such as PI4 kinase, PKC, MAP kinase or c-Src (1). LY294002 has been shown to block PI3 kinase-dependent Akt phosphorylation and kinase activity. Applications: Sutiable for use in vitro and in vivo. Recommended Dilutions: Cultured cells: Recommend treating the cells with LY294002 for one hour prior to, and for the duration of, the stimulation. Optimal dilutions to be determined by researher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Light sensitive. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.