Lyme Disease, Human, IgG, BioAssay(TM) ELISA Kit

The United States Biological Lyme Disease, Human, IgG, BioAssay(TM) ELISA Kit is designed to detect IgG class antibodies to B. burgdorferi in human sera. Wells of plastic microwell strips are sensitized by passive absorption with B. burgdorferi antigen.\n\nKit Components:\nL8001-20A: Microtiter Plate: 1x96wells\nL8001-20B: IgG, Goat x human (HRP): 1x15ml\nL8001-20C: Positive Control: 1x350ul\nL8001-20D: Calibrator: 1x500ul\nL8001-20E: Negative Control: 1x350ul\nL8001-20F: Sample Diluent: 1x30ml\nL8001-20G: Tetramethylbenzidine (TMB): 1x15ml\nL8001-20H: Stop Solution:1x15ml (1N H2SO4, 0.7N HCl)\nL8001-20J: Wash Buffer, 10X: 1x100ml\n \nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L8001-20

Size

1Kit

Applications

ELISA

References

1. Steere, A.C., Taylor, E., Wilson, M.L., Levine, J.F., and Spielman, A.: Longitudinal assessment of the clinical and epidemiologic features of Lyme disease in a defined population. J. Infect. Dis. 154: 295-300 (1986). 2. Rosenfeld, M.E.A.: Serodiagnosis of Lyme disease. J. Clin. Microbiol. 31: 3090-3095 (1993). 3. Steere, A.C., et al., New Engl. J. Med. 308: 733 (1983). 4. Bakken, L.L., et al., J. Clin. Microbiol. 35: 537-543 (1997). 5. U.S. Department of Labor, Occupational Safety and Health Administration: Occupational Exposure to Bloodborne Pathogens. Final Rule. Fed. Register 56: 64175-64182 (1991). 6. Procedures for the collection of diagnostic blood specimens by venipuncture - Second edition: Approved Standard (1984). Published by National Committee for Clinical Laboratory Standards. 7. Procedures for the Handling and Processing of Blood Specimens. NCCLS Document H18-A, Vol. 10, No. 12, Approved Guideline (1990). 8. Russel, H., Sampson, J.S., Schmid, G.P., Wilkinson, H.W., and Plikaytis, B.: Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J. Infect. Dis. 149: 465 (1984). 9. Magnarelli, L.A., et al., J. Infect. Dis. 156: 183-188 (1987). 10. Hunter, E.F., et al., Sex. Trans. Dis. 3: 236 (1986). 11. Steere, A.C., et al., Ann. Intern. Med. 99: 22 (1983). 12. Craft, J.E., et al., Yale J. Biol. Med. 57: 561 (1984). 13. Shrestha, M., et al., Am. J. Med. 78: 235 (1985). 14. Reik, L., et al., Neurology 35: 267 (1985). 15. Barbour, A.:Laboratory Aspects of Lyme Borreliosis. Clin Micr. Rev. 1: 399-414 (1988).