Lyme Disease, Human, IgG, IgM, BioAssay(TM) ELISA Kit

The United States Biological Lyme Disease, Human, IgG, IgM, BioAssay(TM) ELISA Kit is designed to detect IgM and IgG class antibodies to Borrelia burgdorferi in human sera. Wells of plastic microwell strips are sensitized by passive absorption with Borrelia burgdorferi antigen. \nThe test procedure involves three incubation steps.\n1. Test serum samples (properly diluted) are incubated in antigen coated microwells. Any antigen specific antibody in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components. \n2. Peroxidase Conjugated goat anti-sample IgM/IgG is added to the wells and the plate is incubated. The conjugate will react with IgM and/or IgG antibody immobilized on the solid phase in step 1. The wells are washed to remove unreacted conjugate. \n3. The microwells containing immobilized peroxidase conjugate are incubated with peroxidase Substrate Solution. Hydrolysis of the Substrate by peroxidase produces a color change. After a period of time the reaction is stopped and the color intensity of the solution is measured photometrically. The color intensity of the solution depends upon the antibody concentration in the original test sample. \n\nKit Components:\nL8001-22A: Microtiter Plate: 1x96wells\nL8001-22B: IgG, IgM, Goat x human (HRP): 1x15ml\nL8001-22C: Positive control: 1x350ul\nL8001-22D: Calibrator: 1x500ul\nL8001-22E: Negative Control: 1x350ul\nL8001-22F: Sample Diluent: 1x30ml\nL8001-22G: Tetramethylbenzidine (TMB): 1x15ml\nL8001-22H: Stop Solution:1x15ml (1N H2SO4, 0.7M HCl)\nL8001-22J: Wash Buffer, 10X: 1x100ml\n \nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L8001-22

Size

1Kit

Applications

ELISA

References

1. Steere, A.C., et al: J. Infect. Dis. 154:.295-300 (1986). 2. Rosenfeld, M.E.A.:Serodiagnosis of Lyme disease. J. Clin. Microbiol. 31:3090-3095 (1993). 3. Steere, A.C., et al: The Spirochetal Etiology of Lyme Disease. N. Engl. J. Med. 308: 733-740 (1983). 4. Bakken, L.L., et al., J. Clin. Microbiol. 35: 537-543 (1997). 5. Barbour, A.: Laboratory Aspects of Lyme Borreliosis. Clin Micr. Rev. 1: 399-414 (1988). 6. Russel, H., et al., J. Infect. Dis. 149: 465 (1984). 7. Hunter, E.F., et al., Sex. Trans. Dis. 13: 236 (1986). 8. Shrestha, M., et al., Am. J. Med. 78: 235 (1985). 9. Steere, A.C., et al., Ann. Intern. Med. 99:22 (1983). 10. Craft, J.E., et al., Yale J. Biol. Med. 57:561 (1984). 11. Dammin, G.J.: Lyme Disease: Its transmission and diagnostic features. Lab Mgmt. 24:33 (1986). 12. Steere, A.C., et al., Yale J. Biol. Med. 57:453 (1984). 13. Reik, L., et al., Neurology 35: 267 (1985). 14. Procedures for the collection of diagnostic blood specimens by venipuncture: NCCLS Procedure H3; Approved Standard. 15. Procedures for the Handling and Processing of Blood Specimens. NCCLS Document H1, Approved Guideline. 16. U.S. Department of Labor, Occupational Safety and Health Administration. Final Rule; 21CFR 1910.1030.