Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)

LEF1/TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors which consists of four members: Lymphoid enhancer factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3 and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and are downstream factors in the Wnt signaling pathway. LEF1/TCF bind to Wnt response elements to provide a docking site for beta-catenin which translocates to the nucleus and promotes the transcription of target genes upon activation of Wnt signaling (3). LEF1/TCF proteins are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers such as colon cancers (4,5).\nLEF1 has several isoforms due to alternative splicing. LEF1 also has an alternative promoter that is preferentially active in lymphocytes. The isoforms generated by this alternative promoter have no amino-terminal beta-catenin binding domain and may function in a dominant negative manner (6-8).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nFlow Cytometry: 1:200\nImmunofluorescence (IF-IC): 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L8005-10

Size

100ul

Applications

FC, ICC, IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Waterman, M.L., Cancer Metastasis Rev. 23: 41-52 (2004). 2. Schilham, M.W. & Clevers, H., Semin. Immunol. 10: 127-132 (1998). 3. Brantjes, H., et al., Biol. Chem. 383: 255-261 (2002). 4. Reya, T. & Clevers, H., Nature 434: 843-850 (2005). 5. Logan, C.Y. & Nusse, R., Annu. Rev. Cell Dev. Biol. 20: 781-810 (2004). 6. Hovanes, K., et al., Nucleic Acids Res. 28: 1994-2003 (2000). 7. Hovanes, K., et al., Nat. Genet. 28: 53-57 (2001). 8. Kobielak, A., et al., Acta. Biochim. Pol. 48: 221-226 (2001).

Additional Info

Recognizes endogenous level of total human LEF1 protein at ~25-58kD. Does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. \nSpecies Crossreactivity: Mouse, rat.