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Lysosome Associated Membrane Protein 1 (LAMP 1, CD107a, Igp120, IgpA)

Cat no: L9190-08

Lysosome Associated Membrane Protein 1 (LAMP 1, CD107a, Igp120, IgpA)

CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles.1 It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion.2 CD107a is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice.3 LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases.4\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoaffinity chromatography, Immunoprecipitation, Western Blot and Immunoelectron microscopy. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: (same/less than)1ug/10e6 cells\nImmunohistochemistry: Frozen sections\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L9190-08

Size

500ug

Applications

FC, IHC, IP, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in 100mM borate buffered saline, pH 8.0, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified

Isotype

IgG2a,k

References

1. Chen, J.W., W. Pan, M.P. d

Additional Info

Recognizes mouse CD107a/LAMP-1 (Mr 110-140kD with a core protein of Mr ~40kD).

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