Lysosome Associated Membrane Protein 2 (LAMP 2, Igp2, Igp110, CD107b, LAMP2C, LAMPB, Lysosome Associated Membrane Glycoprotein 2 Precursor, MAC3)

Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology. They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane. Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine based motif (YXXI). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome. This stimulation has also been shown to have an associated release of histamine, leukotriene C and prostaglandin D, which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells. They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L9190-12D

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Gozuacik, D., et al., (2004), \"Autophagy as a cell death and tumor suppressor mechanism\", Oncogene, 23: 2891-2906. 2. Kisen G.O., et al., (1993), \"Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells\", Carcinogenesis, 14: 2501-2505. 3. Kamada, Y., et al., (2000), \"Tor-mediated induction of autophagy via Apg1 protein kinase complex\", J. Cell Biol., 150: 1507-1513. 4. Granger, B.L., et al., (1990), \"Characterization and cloning of the Igp110, a lysosomal glycoprotein from mouse and rat cells\", J. Biol. Chem., 265: 12036-12043. 5. Tanaka, Y., et al., (2000), \"Accumulation of autophagic vacuoles and cardiopathy in LAMP-2-deficient mice\", Nature, 902-906. 6. Nishino, I., et al., (2000), \"Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease)\", Nature, 906-910. 7. Gough, N.R., et al., (1995), \"The family of LAMP-2 proteins arises by alternative splicing from a single gene: characterization of the avian LAMP-2 gene and identification of mammalian homologs of LAMP-2b and LAMP-2c\", DNA Cell Biol., 14: 863-867.

Additional Info

Recognizes human LAMP-2 at ~115kD. Species Crossreactivity: mouse.