Lysostaphin, Recombinant

Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.\n\nBiological Activity:\nDetermined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH 8.0, 30 degrees C. \n\nSpecific Activity:\nDetermined to be ~3500 units/mg\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of ~2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis RP-HPLC, using a calibrated solution of rLysostaphin as a Reference Standard.\n\nStorage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L9191-25

Size

1mg

Form

Supplied as a lyophilized powder with no additives.\nReconstitute with 20mM sodium acetate, pH 4.5.\nNote: For a stock solution it is recommended to work with 10mg/ml Lysostaphin in 10mM sodium acetate, pH 4.5.\nNote: For reaction buffer it is recommended to work with 200mM Tris-HCl, pH 8.0.

Purity

(same/more than)93% determined by RP-HPLC

References

1. Koehl, J.L., et al., Antimicrob. Agents Chemother. 48(10): 3749-3757 (2004). 2. Shah, A. & Mond, J., Antimicrob. Agents Chemother. 48(7): 2704-2707 (2004). 3. Heath Farris, M., et al., FEMS Microbiol. Lett. 228(1): 115-119 (2003). 4. Mitra, A., et al., Transgenic Res. 12(5): 597-605 (2003). 5. von Eiff, C., et al., Antimicrob. Agents Chemother. 47(11): 3613-3615 (2003). 6. Wu, J.A., et al., Antimicrob. Agents Chemother. 47(11): 3407-3414 (2003).

Alternative Names

EC=3.4.24.75