Lysozyme, Chicken, RZ3 (Muramidase)

Lysozyme (muramidase) hydrolyzes preferentially the beta-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. A somewhat more limited activity is exhibited towards chitin oligomers.\n\nLysozyme is of widespread distribution in animals and plants. That which has been most extensively studied is from hen egg white (lysozyme C). Lysozyme is also found in mammalian secretions and tissues, saliva, tears, milk, cervical mucus, leukocytes, kidneys, etc.\n\nLysozyme is inhibited by surface-active reagents such as dodecyl sulfate, alcohols and fatty acids. Imidazole and indole derivatives are inhibitors via formation of change-transfer complexes.\n\nUseful for the rapid isolation of RNA from gram negative bacteria. Can be used to prepare small amounts of plasmid DNA.\n\nSolubility: \nDissolves readily at 5mg/ml in 0.1M potassium phosphate, pH 6.24 to give a clear, colorless and complete solution.\n\nActivity: \n(same/more than) 20,000u/mg\n\npH: \n3.0-4.5\n\nAsh: \n(same/less than) 2%\n\nStability: \nLysozyme stored as a dry lyophilized or crystalline powder at 4 degrees C is stable for years. Solutions at pH 4-5 are stable for several weeks refrigerated and for days at ambient temperatures.\n\nUnit Definition:\nThat amount of enzyme causing a decrease in extinction at 450nm of 0.001 per minute at 25 degrees C and pH 6.24 with Micrococcus lysodeikitus as a substrate.\n\nAssay Method:\nThe rate of lysis of Micrococcus lysodeikitus, catalyzed by Lysozome, is proportional to the decrease in extinction at 450nm.\n\nReagents:\n0.1M Potassium phosphate, pH 7.0 \nMicrococcus lysodeikticus cells: Prepare by suspending 9mg of dried Micrococcus lysodeikticus cells in 25ml of 0.1M potassium phosphate buffer, pH 7.0. Dilute to a final volume of 30ml with the same buffer.\n\nDissolve the enzyme at a concentration of 1mg/ml in cold reagent grade water. Keep cool until assay. Immediately prior to assay, dilute to a concentration of 150

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