Lysozyme, Egg white (Muramidase)

Allergy to chicken egg or proteins is one of the more frequent causes of food hypersensitivity in infants and young children. Both IgG and IgA class antibodies may be detected. Ovalbumin intolerance has been implicated in a number of conditions affecting children. Conditions related to ovalbumin intolerance usually resolve once egg and egg based foods have been withdrawn from the patient's diet. Intolerance to egg proteins could be due not only to the ovalbumin protein found in egg white but also to other major proteins present in the yolk. The major proteins of chicken eggs are: Ovalbumin (45kD, 54%), Conalbumin (13%, 80kD), Ovomucoid (11%, 28kD), Lysozyme (3.5%, 14kD), Globulins (G2, G3) (8.0%), Ovomucin (1.5%). Other protein components include, flavoprotein (0.8%), ovoglycoprotein (0.5%), ovomacroglobulin (0.5%), ovoinhibitor (0.1%) and avidin (0.05%). Lysozyme is a single chain polypeptide of 129aa cross-linked with four disulfide bridges. It hydrolyzes b-1-4 linkages between N-acetylmuraminic acid and N-acetyl-D- glucosamine residues in peptidoglycan and between N-acetyl-D- glucosamine residues in chitodextrin. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed more easily in the presence of EDTA.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-1:50,000. \nWestern Blot: 1:1000-1:5000 using Chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L9200-05B

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Ch/Bird

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.

P Type

Pab

Purity

Serum.

Isotype

IgG

References

1. Rupley J A(1964) BBA 83, 245-255; 2. Holler H (1975) Biochem. 14, 2377; 3. Abgar S (2000) J. Biochem. 267, 5916-5925; 4. Canfiled RE (1963) JBC 238, 2698-2707.

Additional Info

Recognizes chicken Lysozyme, Egg white (Muramidase).