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LYVE1 (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1, LYVE-1, Cell Surface Retention Sequence-binding Protein 1, CRSBP-1, Extracellular Link Domain-containing Protein 1, Hyaluronic Acid Receptor, CRSBP1, HAR, XLKD1, UNQ230/PRO263) (APC)

Cat no: L9375-01B

LYVE1 (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1, LYVE-1, Cell Surface Retention Sequence-binding Protein 1, CRSBP-1, Extracellular Link Domain-containing Protein 1, Hyaluronic Acid Receptor, CRSBP1, HAR, XLKD1, UNQ230/PRO263) (APC)

LYVE1, also named extracellular link domain containing 1, is a 60-70kD type I transmembrane protein that functions as a receptor for both soluble and immobilized hyaluronan (HA). It shares 41% aa sequence homology with CD44, another known receptor for HA. LYVE1 expression is mostly restricted to lymphatic vessels. LYVE1 is a useful molecular marker for lymphatic endothelial cells.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul labels up to 1x10e6 cells. Validated for use on HUVEC cells.\nOptimal dilutions to be determined by the researcher.\n\nFlow Cytometry Protocol:\n1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.\n2. After blocking, add 10ul of L9375-01B to up to 1x10e6 cells and incubate for 30 minutes at RT. \n3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.\n4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG1 antibody. \n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for at 12 months at 4 degrees C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

L9375-01B

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 1ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human LYVE-1.

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