The M2-10B4 cell line is a clone derived from bone marrow stromal cells from a (C57BL/6J X C3H/HeJ)F1 mouse. The cells express laminin and collagen IV, but do not express collagen I or Factor VIII. The cells will support human and murine myelopoiesis in long term culture, and will support certain murine stromal cell dependent pre-B cell lines in vitro. The species was confirmed by Real-Time PCR