Macrophage Colony Stimulating Factor, Recombinant, Human (M-CSF, CSF-1, Lanimostim, Macrophage colony-stimulating factor 1 precursor, MCSF, M-CSF, MGC31930)

Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs).A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo.\n\nRecombinant Human M-CSF is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8kD.\n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser. \n\nBiological Activity:\nM-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 5ng/ml, corresponding to a Specific Activity of 2 x 10e5 IU/mg. \n\nEndotoxin:\nLess than 0.1ng/ug (IEU/ug) of M-CSF. \n\nStorage and Stability: \nStore powder dessicated at -20C. Liquid can be stored at 4C for 2-7 days, long term at -20C. For long term it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to prevent repeated freeze-thaw cycles.\n

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

M1201A

Size

2ug

Form

Supplied as a lyophilized powder in HSA, Manntiol. Reconstitute sterile ddH2O, 0.1% HSA or BSA to (same/more than)100ug/ml, which can then be further diluted to other aqueous solutions

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: (same/less than) 0.1ng/ug (IEU/ug)

References

1. SHIP2 is recruited to the cell membrane upon macrophage colony-stimulating factor (M-CSF) stimulation and regulates M-CSF-induced signaling. Wang Y, Keogh RJ, Mitchell CA, Schurmans S, J Immunol 2004 Dec 1;173(11):6820-30 2. Serum levels of matrix metalloproteinase 3 and macrophage colony-stimulating factor 1 correlate with disease activity in ankylosing spondylitis. Yang C, Gu J, Baeten D, Maksymowych WP, Arthritis Rheum 2004 Oct 15;51(5):691-9 3. Synergistic effect of interleukin (IL)-1alpha and ceramide analogue on the production of IL-6, IL-8, and macrophage colony-stimulating factor by endometrial stromal cells. Kawano Y, Fukuda J, Matsumoto H, Fertil Steril 2004 Oct;82 Suppl 3:1043-7 4. Membrane-bound macrophage colony-stimulating factor mediated auto-juxtacrine downregulates matrix metalloproteinase-9 release on J6-1 leukemic cell. Rao Q, Zheng GG, Lin YM, Exp Biol Med (Maywood) 2004 Oct;229(9):946-53 5. Preoperative sensitivity and specificity for early-stage ovarian cancer when combining cancer antigen CA-125II, CA 15-3, CA 72-4, and macrophage colony-stimulating factor using mixtures of multivariate normal distributions. Skates SJ, Horick N, Xu FJ, van der Zee AG, J Clin Oncol 2004 Oct 15;22(20):4059-66 6. Discrimination of heterozygous from wild-type mice bearing a mutation in the macrophage-colony-stimulating factor gene using capillary electrophoresis. Techel D, Bergmann C, Jost MM, Lab Anim (NY) 2004 Sep;33(8):33-5.