Macrophage Inflammatory Protein 1 beta, Recombinant, Human (MIP-1b, CCL4)

Recombinant Human MIP-1 is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7620 Dalton.\n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Met-Gly-Ser.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity:\nrHuMIP-1 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human blood monocytes using a concentration of 5-20ng/ml.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of rHuMIP-1.\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280nm. \n2. Analysis by RP-HPLC, using a standard solution of MIP-1 as a Reference Standard.\n\nReconstitution:\nReconstitute the lyophilized rHuMIP-1 in sterile 18MOmega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M1202-32A

Size

2ug

Form

Supplied as a lyophilized powder. No additives.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Glycosaminoglycan disaccharide alters the dimer dissociation constant of the chemokine MIP-1 beta. McCornack MA, Boren DM, Biochemistry 2004 Aug 10;43(31):10090-101 2. [Serum and cerebrospinal fluid concentration of inflammatory proteins MIP-1-alpha and MIP-1-beta and of interleukin 8 in the course of borreliosis] Grygorczuk S, Pancewicz S, Swierzbinska R, Neurol Neurochir Pol 2003 Jan-Feb;37(1):73-87 3. Levels of macrophage inflammatory protein 1 alpha (MIP-1 alpha) and MIP-1 beta in intervillous blood plasma samples from women with placental malaria and human immunodeficiency virus infection. Chaisavaneeyakorn S, Moore JM, Othoro C, Nahlen BL, Clin Diagn Lab Immunol 2003 Jul;10(4):631-6 4. Human neuronal cells (NT2-N) express functional substance P and neurokinin-1 receptor coupled to MIP-1 beta expression. Li Y, Douglas SD, Lai J, Ho W, J Neurosci Res 2003 Feb 15;71(4):559-66 5. Characterization of the role of the N-loop of MIP-1 beta in CCR5 binding. Bondue A, Jao SC, Parmentier M, Biochemistry 2002 Nov 19;41(46):13548-55 6. [Concentration of macrophage inflammatory proteins MIP-1 alpha and MIP-1 beta and interleukin 8 in erythema migrants] Grygorczuk SS, Pancewicz S, Zajkowska J, Pol Merkuriusz Lek 2002 Jun;12(72):458-61.