Macrophage Inflammatory Protein 1

Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells. \n\nApplications:\nSuitable for use in ELISA and neutralization. Other applications not tested.\n\nRecommended Dilution:\nELISA: 2-4ug/ml In a sandwich ELISA (assuming 100ul/well), a concentration of 1-2ug/ml will detect (same/more than) 2000pg/well of recombinant MIP-1a when used with M1202-25C as the detection antibody.\nNeutralization: 5-10ug/ml, to yield one-half maximal inhibiton [ND50] of the biological activity (100ng/ml)\nOptimal dilution determined by the researcher. \n\nHybridoma:\nProduced in Balb/c X ICR F1 mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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