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Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (BSA & Azide Free)

Cat no: M1202-74X

Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (BSA & Azide Free)

L1 Protein or Calprotectin Molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin/paraffin): 1:1000 for 20 minutes at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml in PBS for 10 minutes at 37 degrees C.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nTonsil, lymph node or spleen\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M1202-74X

Size

100ug

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Fel, GP, NHP, Prc

Form

Supplied as a liquid in 10mM PBS, pH 7.4. No preservatives added. No stabilizing proteins added. Also available with BSA and azide. See M1202-74.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1.

Additional Info

Recognizes human neutrophils, monocytes, macrophages and squamous mucosal epithelia. Has been shown as an important marker for identifying macrophages in tissue sections. Species Crossreactivity: baboon, monkey, porcine, feline, canine, guinea pig, rat and mouse.

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