Macrophage, Monocyte Chemotactic Protein 3, Recombinant, Mouse (MCP-3, MARC)

Recombinant Murine MCP-3 produced in E. coli is a non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8510 Dalton. \n\nBiological Activity: Murine MCP-3 is fully biologically active when compared to standard. The specific activity as determined by the ability of Murine MCP-3 to chemoattract Balb/C mouse spleen MNCs at 10-100ng/ml.\n\nAA Sequence:\nQPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

M1203-30A

Size

2ug

Form

Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Purity

(same/more than) 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.

References

1. Th2- and to a lesser extent Th1-type cytokines upregulate the production of both CXC (IL-8 and gro-alpha) and CC (RANTES, eotaxin, eotaxin-2, MCP-3 and MCP-4) chemokines in human airway epithelial cells. Int Arch Allergy Immunol 2003 Aug;131(4):264-71\n2. [Active immunity for anti-colorectal cancer induced by chemokine MCP-3 gene transfection] Ai Zheng 2002 May;21(5):504-8\n3. Transfection of colorectal cancer cells with chemokine MCP-3 (monocyte chemotactic protein-3) gene retards tumor growth and inhibits tumor metastasis. World J Gastroenterol 2002 Dec;8(6):1067-72\n4. Gelatinase B, PECAM-1 and MCP-3 gene polymorphisms in Belgian multiple sclerosis. Neurol Sci 2002 Aug 15;200(1-2):43-8\n5. Eotaxin but not MCP- 3 induces eosinophil influx into nasal fluid in allergic patients. Allergy 2002 Jun;57(6):519-28\n6. Transduction of human MCP-3 by a parvoviral vector induces leukocyte infiltration and reduces growth of human cervical carcinoma cell xenografts. J Gene Med 2001 Jul-Aug;3(4):326-37