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Magic™ Anti-GFP tag antibody, clone 6G23.5 [eFluor® 660]

Cat no: DMAB22993

Magic™ Anti-GFP tag antibody, clone 6G23.5 [eFluor® 660]

Rat anti-GFP tag monoclonal antibody for FC. Green fluorescence protein (GFP) is a38kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

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SPECIFICATIONS

Catalog Number

DMAB22993

Hosts

Rat

Clone

6G23.5

Target

GFP tag

Isotype

IgG2a

Clonality

Monoclonal

Storage Temp

-20В°C, avoid repeated freeze/thaw cycles

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