Magic™ Anti-PhosphoT (Phospho T) monoclonal antibody, clone SN203

Rabbit Anti-PhosphoT (Phospho T) monoclonal antibody for WB, IP, ICC, IHC, FC, ELISA. Phosphorylation of threonine residues is associated with manygrowth factors and oncogene protein kinases, and is important for cellsignaling in activation, proliferation and differentiation. Proteinphosphorylation and dephosphorylation are basic mechanisms for themodification of protein function in eukaryotic cells. Phosphorylation is arare post-translational event in normal tissue, however, the abundance ofphosphorylated cellular proteins increases several fold following variousactivation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signaltransduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residuesupon binding of ligands to their receptors. T cell antigen receptor complexor the receptors for some hemopoietic growth factors may stimulate thesephosphorylation associated kinases, and cells transformed by viral oncogenescontain elevated levels of phosphorylated tyr/ser/thr. Immunoblotting ofcellular proteins with antibodies directed against phosphoamino acids isadvantageous as it does not involve 32P labeling, and can therefore beemployed to monitor alterations in phosphorylation of specific proteins asthey occur in intact organs or the whole animal. Indeed, mono and polyclonalantibodies directed against phosphorylated residues have been generated andfound useful as analytical and preparative tools because they enable therapid identification, quantification and immunoaffinity isolation ofphosphorylated cellular proteins.

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