Malachite Green Phosphate Assay Kit, BioAssay(TM)

Description:\nThe Malachite Green Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be executed in tubes, cuvettes or multi-well plates. The assays can be conveniently performed in 96- and 384-well plates for high-throughput screening of enzyme inhibitors. \n\nKey Features:\nReagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 1.6 pmoles of phosphate and useful range between 0.02uM and 40uM phosphate. Fast and convenient: homogeneous "mix-and-measure" assay allows quantitation of free phosphate within 20 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z' factors of 0.7 to 0.9 are observed in 96- well and 384-well plates. Can be readily automated on HTS liquid handling systems. \n\nApplications:\nPhosphatase Assays: liberation of phosphate from peptide, protein or small molecule substrate. Lipase Assays: liberation of phosphate from phospholipids Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc). Quantitation of Phosphate in phospholipids, proteins and DNAs, etc. Drug Discovery: high-throughput screen for phosphatase inhibitors. \n\nKit Contents (2,500 Assays in a 96-Well Plate:\nReagent A: 50ml Reagent B: 1ml Standard: 1ml 1mM phosphate Storage conditions. The reagents and standard are stable for one year when stored at 4 degrees C. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.

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