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Malate BioAssay(TM) Kit

Cat no: M2115-03

Malate BioAssay(TM) Kit

L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during\ncarbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic\nfermentation with the formation of CO2. Malate is frequently used as an additive in the food\nand pharmaceutical industries, so quantitating malic acid is important in manufacturing beer,\nwine, cheese and fruits, among others. This is an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (lambda-max = 450nm). The assay can detect 1~35nmol of Malate in a 50ul sample with a detection sensitivity ~20uM.\n\nKit Components:\nMalate *Assay Buffer 1x20ml\nMalate Enzyme Mix lyophilized\nWST Substrate lyophilized\nMalate Standard (10umol) lyophilized\n\nStorage and Stability:\nStore the kit at -20 degrees C, protect from light. Allow *Assay Buffer to warm to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2115-03

Size

100Tests

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