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Malate Dehydrogenase 1, 1-334aa, Recombinant, Human (MDH1, Cytosolic Malate Dehydrogenase)

Cat no: M2115-35B

Malate Dehydrogenase 1, 1-334aa, Recombinant, Human (MDH1, Cytosolic Malate Dehydrogenase)

MDH1 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. This protein also regulates p53-dependent cell-cycle arrest and apoptosis in response to glucose deprivation.\n\nSource:\nRecombinant corresponding to aa1-334 of human recombinant MDH1 protein, fused to His-tag at C-terminus, expressed in E.coli.\n\nAA Sequence:\nMSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSM\nPRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN\nVIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG\nVISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2115-35B

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol.

Purity

~95% (SDS-PAGE)

References

1. Youn HD., et al. (2009) Cell Death Differ. 16(5):738-48. 2. Waye MM., et al (2005) J Cell Biochem. 94(4):763-73.

Read more on Supplier website

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info
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