MAP Kinase Assay Kit, BioAssay(TM)

Supplier:

United States Biological

Cat no: M2353

MAP Kinase Assay Kit, BioAssay(TM)

The assay kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates and column fractions. Crude cell lysates may also be used but detergents/biochemicals contained in the cell lysis buffer may inhibit MAP Kinase activity. Furthermore, although three inhibitors are included with the kit, editors may suggest other unknown kinases found in crude lysates are responsible for MBP phosphorylation. The assay kit is based on phosphorylation of a specific substrate (myelin\nbasic protein, MBP). The phosphorylated substrate is then analyzed by immunoblot analysis, probing with a monoclonal Phospho-specific MBP antibody. The enzyme assay is rapid, convenient and specific for MAP Kinase.\n\nKit Components:\nM2353A: Assay Dilution Buffer 2x1ml\nM2353B: Mab Myelin Basic Protein, phosphorylated (MBP) 1x100ug \nM2353C: Magnesium/ATP Cocktail 2x1ml\nM2353D: MAP Kinase Substrate Cocktail II: 2x1ml\nM2353E: MAP Kinase Inhibitor Cocktail: 1x1ml\n\nStorage and Stability:\nStore other components at -20C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2353

Size

1Kit

Applications

WB

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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