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MAP Kinase Kinase 3, phosphorylated (Ser189) (Dual Specificity Mitogen-activated Protein Kinase Kinase 3, Mitogen-activated Protein Kinase Kinase 3, MAP2K3, MAPKK 3, MKK3, MAPK/ERK Kinase 3, MEK 3, MEK3, PRKMK3, Stress-activated Protein Kinase Kinase 2, S

Cat no: M2363-10L

MAP Kinase Kinase 3, phosphorylated (Ser189) (Dual Specificity Mitogen-activated Protein Kinase Kinase 3, Mitogen-activated Protein Kinase Kinase 3, MAP2K3, MAPKK 3, MKK3, MAPK/ERK Kinase 3, MEK 3, MEK3, PRKMK3, Stress-activated Protein Kinase Kinase 2, S

MKK3 is a member of the MAPK kinase (MAPKK) superfamily. MKK3 protein kinase is a critical component of a tumor necrosis factor-stimulated signaling pathway that causes increased expression of inflammatory cytokines. The protein specifically activates p38/MPK2. Activation of the p38 pathway can induce a number of cellular responses, including alterations in transcription, the cell cycle, inflammation, and apoptosis. The protein is present in all tissues and enriched in skeletal muscle. Pathological role of the protein has been identified in colon cancer. Human MKK3 gene is localized on chromosomal region, 17q11.2.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMDA-MB-435 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2363-10L

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tetsuo, M. et al. J. Biol. Chem. 271:13675-13679 (1996). 2. Mark, W. et al. Cell Biology. 96:3763-3768 (1999).

Additional Info

Recognizes human MKK3, phosphorylated at Ser189. Species Crossreactivity: rat.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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