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MAP Kinase Kinase 3, Recombinant, Human, aa1-318, His-tag (Dual Specificity Mitogen-activated Protein Kinase Kinase 3, Mitogen-activated Protein Kinase Kinase 3, MAP2K3, MAPKK 3, MKK3, MAPK/ERK Kinase 3, MEK 3, MEK3, PRKMK3, Stress-activated Protein Kinas

Cat no: M2865-90Z

MAP Kinase Kinase 3, Recombinant, Human, aa1-318, His-tag (Dual Specificity Mitogen-activated Protein Kinase Kinase 3, Mitogen-activated Protein Kinase Kinase 3, MAP2K3, MAPKK 3, MKK3, MAPK/ERK Kinase 3, MEK 3, MEK3, PRKMK3, Stress-activated Protein Kinas

MAP2K3, also known as dual specificity mitogen-activated protein kinase kinase 3, is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter.\n\nSource: \nRecombinant corresponding to aa1-318 from human MAP2K3, fused to His-tag at N-terminus expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MSKPPAPNPT PPRNLDSRTF ITIGDRNFEV EADDLVTISE LGRGAYGVVE KVRHAQSGTI MAVKRIRATV NSQEQKRLLM DLDINMRTVD CFYTVTFYGA LFREGDVWIC MELMDTSLDK FYRKVLDKNM TIPEDILGEI AVSIVRALEH LHSKLSVIHR DVKPSNVLIN KEGHVKMCDF GISGYLVDSV AKTMDAGCKP YMAPERINPE LNQKGYNVKS DVWSLGITMI EMAILRFPYE SWGTPFQQLK QVVEEPSPQL PADRFSPEFV DFTAQCLRKN PAERMSYLEL MEHPFFTLHK TKKTDIAAFV KEILGEDS \n\nMolecular Weight: \n~38.3kD (338aa), confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2865-90Z

Size

10ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Rampoldi L., et al. (1997) Cytogenet Cell Genet. 78(3-4):301-3. 2. Barr AJ., et al. (2002) Biochem Biophys Res Commun. 293(1):647-52.

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