MAP Kinase Kinase 6 (Dual Specificity Mitogen-activated Protein Kinase Kinase 6, MAP2K6, MAPKK 6, MAPKK6, MKK6, PRKMK6, MAPK/ERK Kinase 6, MEK 6, MEK6, Stress-activated Protein Kinase Kinase 3, SAPK Kinase 3, SAPKK3, SAPKK-3, SKK3)

MAP kinase kinase 6 (Mek6/MKK6) is a 38 kD dual-specificity protein kinase that is abundant in skeletal muscle and is activated by UV light, anisomycin, osmotic shock and inflammatory cytokines, but not by phorbol esters, NGF or EGF (1). Mek6 phosphorylates and activates MAP kinases such as p38 (2,3) and p38 beta (4). Mek6 is phosphorylated and activated by ASK1, a MAPKKK that induces apoptosis when overexpressed (5). Mek6 also interacts with mixed lineage kinase-3 (MLK-3), a kinase that participates in stress response kinase cascade, possibly by phosphorylating MKK4, an isoform of Mek6 (6).\n \nApplications: \nWestern Blot (Colorimetric): 4ug/ml \nImmunoprecipitation: 4ug/ml \nOptimal dilutions to be determined by researcher.\n \nPositive Controls:\nHeLa Cell Lysate (Heat Shocked), \nRat Brain Tissue Extract, \nMouse Brain Tissue Extract

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SPECIFICATIONS

Catalog Number

M2363-22B

Size

25ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Rabbit, Xen/Amph

Form

Supplied as a liquid in borate buffered saline, pH 8.2 25mM sodium borate, 100mM boric acid, 75mM sodium chloride, 5mM EDTA (disodium salt).

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Stein, B., Brady, H., Yang, M.X., Young, D.B., Barbosa, M.S. (1996) J. Biol. Chem. 271: \n11427-11433 \n2. Raingeaud, J. Whitmarsh A.J., Barrett T., Derijard B., Davis R.J. (1996) Mol. Cell Biol. 16: \n1247-1255 \n3. Cuenda, A., Cohen P., Buee-Scherrer V., Goedert M. (1997) EMBO J. 16: 295-305 \n4. Jiang, Y., Chen C., Li Z., Guo W., Gegner J.A., Lin S., Han J. (1996) J. Biol. Chem. 271: \n17920-17926 \n5. Ichijo, H. Nishida E., Irie K., ten Dijke P., Saitoh M., Moriguchi T., Takagi M., Matsumoto \nK., Miyazono K., Gotoh Y. (1997) Science 275: 90-94 \n6. Tibbles, L. A. Ing Y.L., Kiefer F., Chan J., Iscove N., Woodgett J.R., Lassam N.J. (1996) \nEMBO J. 15: 7026-7035 \n

Additional Info

The rabbit immunoaffinity purified antibody recognizes an ~38kD protein corresponding to the apparent molecular mass of Mek6 on SDS-PAGE immunoblots, in samples of human, rat, mouse and Xenopus origin. Antibody specificity is confirmed by peptide inhibition studies. \n

Alternative Names

Mitogen-activated Protein Kinase Kinase 6, EC=2.7.12.2