MAP Kinase Kinase 6, Recombinant, Human, GST-Tag (Dual Specificity Mitogen-activated Protein Kinase Kinase 6, MAP2K6, MAPKK 6, MAPKK6, MKK6, PRKMK6, MAPK/ERK Kinase 6, MEK 6, MEK6, Stress-activated Protein Kinase Kinase 3, SAPK Kinase 3, SAPKK3, SAPKK-3,

Source:\nRecombinant corresponding to aa2-end of human MKK6 with N-terminal GST tag expressed in a Baculovirus infected Sf9 cell expression system (NM_002758.3).\n\nSpecific Activity:\nEnzyme reaction is conducted in a buffer containing 50mM HEPES, pH7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 100ng of inactive MAPK14, 2uM substrate (SER/THR 4) at RT for 1 hour. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.\n\nApplications: \nSuitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing. Store at -70 degrees C. Aliquots are stable for at least 6 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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