MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)

P38 MAP kinase (MAPK), also called RK (3) or CSBP (2), is the mammalian homologue of the yeast HOG kinase and participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, alpha, beta, gamma and delta, have been identified so far. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8). Nonphosphorylated p38 MAP Kinase Control Cell.\n\nWestern Blots: As controls, we recommend using 15ul of phosphorylated and nonphosphorylated p38 MAP Kinase Control Cell Extracts. Boil for 2 minutes prior to use.\n\nNonphosphorylated p38 MAP Kinase Control Cell Extracts: \nTotal cell extracts from C-6 glioma cells prepared without anisomycin treatment serve as a negative control. \n\nPhosphorylated p38 MAP Kinase Control Cell Extracts:\nTotal cell extracts from C-6 glioma cells prepared with anisomycin treatment serve as a positive control.

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SPECIFICATIONS

Catalog Number

M2352-01C

Size

10 Blots

Applications

WB

Form

Supplied as a liquid in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25 degrees C), 2% w/v SDS, 10% glycerol, 50 mM DTT and 0.01% w/v bromophenol blue.

References

(1) Han, J. et al. (1994) Science 265, 808

Alternative Names

EC=2.7.11.24