MAP Kinase p44/42 (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, Mapk, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MAPK 2, ERK2, PRKM1 /

Mitogen Activated Protein Kinases (MAPKs) play pivotal roles in mediating signal transduction from the cell surface to the nucleus. These kinases are encoded by distinct genes and together form a family of kinases whose activation is dependent upon dual phosphorylation on speci?c threonine and tyrosine residues. In yeast, a number of different MAP Kinases have been identi?ed and are activated by distinct signaling pathways. In mammalian cells, the best characterized sub-group of the MAP Kinase family are the Extracellular Signal Regulated Kinases (ERKs). To date, at least 4 distinct ERKs have been identified including: ERK1 (p44/p43), ERK2 (p42/p43), ERK3 (p62), and ERK4 (p45). Analysis of cDNAs encoding MAP Kinase suggest that numerous other ERKs may exist. MAP Kinase has been shown to phosphorylate numerous proteins\nincluding: RSK (2), c-Fos, c-Jun, c-Myc, c-raf, MAP2, and MEK (3). MAP Kinase is directly activated when phosphorylated by MEK and indirectly stimulated by many factors (4,5,6).\n\nApplications: \nSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml \nImmunoprecipitation (7-9, 13, 14): 2-5ug/reaction \nWestern Blot (1-8, 10-19): 1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nPositive Controls: \nHuman A431 and K562 cells, canine MDCK cells, mouse brain, rat brain and recombinant ERK2.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2360-02K

Size

200ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by immunoaf?nity chromatography.

Isotype

IgG1,k

References

1. Boulton, T., et al., Science 249: 64-66 (1990). 2. Sturgill, T.W., et al., Nature 334: 715-718 (1988). 3. Matsuda, S., et al., J. Biol. Chem. 267: 3277-3281 (1993). 4. Ward, et al., Nature 367: 651-654 (1994). 5. Boulton, T.G., et al., Cell 65: 663-675 (1991). 6. Crews, C.M., et al., Science 258: 478-480 (1992). 7. Hurley, M.M., et al., J. of Bone & Mineral Research 11(9): 1256-1263 (1996). 8. Kazuya I.-P., J. Biol. Chem. 272(45): 28,750-28,756 (1997). 9. Chan, E., et al., J. Clin. Invest. 103: 1337-1344 (1999). 10. Ozaki, I., et al., J. Biol. Chem. 274: 5310-5317 (1999). 11. Lenomand, H., et al., EMBO J. 18: 1869-1877 (1999). 12. Mark, M.D., et al., Journal of Cell Biology 130(1): 701-710 (1995). 13. Brenner, B., et al., PNAS 93: 15,376-15,381 (1996). 14. Webb, C.P, et al., PNAS 95: 8773-8778 (1998) 15. Waddel, T.K., et al., J. Biol. Chem. 270(25): 15,403-15,144 (1995). 16. Mark, M.D., et al., J. Biol. Chem. 272: 17,238-17,244 (1997). 17. Gonowski, A.M., Journal of Cell Biology 269(11): 7874-7878 (1994). 18. Deusbery, N.S., et al., Science 280: 734-737 (1998) 19. Lee, J.R., and Koretzky, G.A., J. Immunology 161(4): 1637-1644 (1998) 20. Marshall, C.W., et al., Cell 80: 179-185 (1995). 21. Herskowitz, I., et al., Cell 80:187-197 (1995). 22. Cobb, M.H., et al., Cell Regulation 2: 965-978 (1991).

Additional Info

Recognizes both rat ERK1 and ERK2. Species Crossreactivity: Human, canine, mouse.

Alternative Names

EC=2.7.11.24