MAP Kinase p44/42, phosphorylated (Thr202, Tyr204), Immunohistochemistry Controls (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activ

p44/42 MAPK (Erk1 and Erk2), SAPK/JNK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation and control of cellular responses to cytokines and stress.p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY*, resulting in p38 activation.\n\nSections are cut freshly upon ordering.\n\nDescription:\nEach control slide contains formalin fixed, paraffin-embedded NIH/3T3 cells, both untreated and treated with Phorbol-13-Myristate-12-Acetate (PMA), that serve as a control for Phospho-p44/42 MAPK (Thr202/Tyr204) immunostaining. PMA induces phosphorylation of p44/42 MAPK. Western Blot analysis was performed on extracts derived from the same cells to verify the efficacy of the the PMA treatment.\n\nApplications:\nThese slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of prodcucts that can be used with these slides.\n\nCompanion Products\n

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SPECIFICATIONS

Catalog Number

M2352-20B

Size

5 Slides

References

1 Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139. 2 Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218. 3 Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444. 4 Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485. 5 Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.