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MAP Kinase p44, p42, phosphorylated (Thr202, Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Ki

Cat no: M2363-01A

MAP Kinase p44, p42, phosphorylated (Thr202, Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Ki

Applications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.05ug/ml.\nWestern Blot: 0.5ug/ml (ECL) Recommended blocking buffer: Casein/Tween-20.\nImmunocytochemistry: 1-10ug/ml\nImmunoprecipitation: 1ug/10e6 cells (Pervanadate treated) A431 orHepG2 cells)\nOptimal dilutions to be determined by the researcher.\n\nPositive control Included:\nH2030-03H1 HepG2 Cell Lysate, Pervanadate Treated.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2363-01A

Size

50ug

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Conjugates

Biotin

Form

Supplied as a liquid in PBS, 0.09% sodium azide, PEG, Sucrose and 40% glycerol. Labeled with Biotin.

P Type

Mab

Purity

Purified by cell culture supernatant by subsequent thiophillic adsorption and size exclusion chromatography.

Isotype

IgG1

Additional Info

Recognizes the FLpTEpYVA motif of activated human ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein. Does not cross-react with activated JNK or p38.\nSpecies Crossreactivity: mouse, rat, and canine.

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