MAPK Family BioAssay(TM) Sample Kit

P44/42 MAPK (Erk1 and Erk2), SAPK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, and the differentiation and control of cellular responses to cytokines and stress. p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* resulting in p38 activation.\n\nMAPK Family Antibody Sampler Kit contains 40ul of each primary antibody (p44/42 MAP Kinase, SAPK/JNK, p38 MAP Kinase) nd 50ul of anti-rabbit IgG secondary antibody labeled with HRP.\n\nKit Components:\nM2352-40A: p44/42 MAP Kinase Antibody 1x40ul\nM2352-40B: SAPK/JNK Antibody 1x40ul\nM2352-40C: p38 MAP Kinase Antibody 1x40ul\nM2352-40D: Rabbit ant-Human IgG, HRP, 1x50ul\n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2352-40

Size

1Kit

Applications

ELISA, WB

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Purity

Purified by protein A and peptide affinity chromatography.

References

1. Lewis, T.S., et al., Adv. Cancer Res. 74: 49-139 (1998). 2. Garrington, T.P., Johnson, G.L., Curr. Opin. Cell. Biol. 11: 211-218 (1999). 3. Schaeffer, H.J., Weber, M.J., Mol. Cell. Biol. 19: 2435-2444 (1999). 4. Whitmarsh, A.J., Davis, R.J., Trends Biochem. Sci. 23: 481-485 (1998). 5. Cobb, M.H., Prog. Biophys. Mol. Biol. 71: 479-500 (1999).

Additional Info

Each antibody recognizes only its specific target. The antibodies do not crossreact with one another.