MAPK Family, phosphorylated BioAssay(TM) Sample Kit

P44/42 MAPK (Erk1 and Erk2), p38 MAPK and SAPK function in protein kinase cascades that play a critical role in the regulation of cell growth and differentiation and the control of cellular responses to cytokines and stress. p44/42 MAPK (Erk1 and Erk2) are activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). MEK1/2 Inhibitor (U0126) inhibits activation of MEK1 and MEK2 with IC50 values of 4uM and 50uM, respectively. SAPK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* resulting in p38 activation. Phospho-MAPK Family Antibody Sampler Kit contains 40ul of each primary antibody [Phospho-p44/42 MAPK (Thr202/Tyr204), Phospho-SAPK/JNK (Thr183/Tyr185), Phospho-p38 MAPK (Thr180/Tyr182)], 50ul of anti-rabbit IgG secondary antibody (HRP conjugated) and 0.3mg of MEK1/2 Inhibitor (U0126).\n\nApplications: \nSuitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern IP IHC ICC ELISA \nPhospho-p44/42 MAPK (Thr202/Tyr204) Ab 1:1000 1:100 1:100 1:250 \nPhospho-SAPK/JNK (Thr183/Tyr185) Ab 1:1000 NT 1:50 1:2000 \nPhospho-p38 MAP Kinase (Thr180/Tyr182) Ab 1:1000 1:50 1:100 1:2000 \n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

M2352-41

Size

3x40ul

Applications

ELISA, ICC, IHC, IP, WB

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

Purity

Purified by protein A and peptide affinity chromatography.

References

(1) Favata, M.F. et al. (1998) J. Biol. Chem. 273, 18623

Additional Info

Recognizes Phospho-p44/42 MAPK, Phospho-SAPK/ JNK and Phospho-p38 MAPK Antibodies only recognize the phosphorylated form of p44/42 MAPK, SAPK/JNK and p38 MAPK, respectively. They do not appreciably crossreact with other MAPK family members.