Matrix Metalloproteinase 13, Recombinant, Human (MMP-13, Collagenase 3)

Latent recombinant human pro-collagenase (MMP-13) also called collagenase-3 truncated from C-terminal. Recombinant Human MMP-13 produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 27kD. Recombinant MMP-13 is purified by proprietary chromatographic techniques.\n\nBiological Activity: \nRecombinant MMP-13 activity is determined by the cleavage of fluorogenic peptide, 100 ng of enzyme activated with APMA will digest 80% fluorogenic peptide substrate (0.1ml of 20microM solution) at 35 degrees C for 30 minutes, (10microg0 assays).\n\nApplications: \nUsed as a standard for assaying MMP-13 or for screening inhibitors.\n\nStability: \nRecombinant MMP-13 although stable at 140C for 1 week, should be stored desiccated below -180C. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

M2428-27D

Size

1ug

Form

Supplied as a liquid in 0.05M Tris-HCl, pH 7.5, 0.2M NaCl, 5mM CaCl2, 20uM ZnSO4 and 0.1% BSA.

Purity

> 90% (SDS-PAGE)

References

1. Epidermal development and wound healing in matrix metalloproteinase 13-deficient mice.\n\nJ Invest Dermatol 2006 Feb;126(2):486-96\n\n2. Increased expression of matrix metalloproteinase-2, matrix metalloproteinase-9 and matrix metalloproteinase-13 in lesional skin of bullous pemphigoid.\n\nInt Arch Allergy Immunol 2006;139(2):104-13\n\n3. Matrix metalloproteinase-13 and membrane type-1 matrix metalloproteinase in peritoneal fluid of women with endometriosis.\n\nGynecol Endocrinol 2005 Aug;21(2):106-10\n\n4. Suppressed TGF-beta1 expression is correlated with up-regulation of matrix metalloproteinase-13 in keloid regression after flashlamp pulsed-dye laser treatment.\n\nLasers Surg Med 2005 Jan;36(1):38-42\n\n5. Altered endochondral bone development in matrix metalloproteinase 13-deficient mice.\n\nDevelopment 2004 Dec;131(23):5883-95\n\n6. Cyclophilin C-associated protein is a mediator for fibronectin fragment-induced matrix metalloproteinase-13 expression.\n\nJ Biol Chem 2004 Dec 31;279(53):55334-40