Matrix Metalloproteinase 2, CT, Hemopexin-like Domain, Recombinant, Human (MMP-2, Collagenase IV, Gelatinase A)

PEX can block angiogenesis and tumor growth in vivo, providing a potentially novel therapeutic approach for diseases associated with neovascularization. The appearance of PEX at sites of neovascularization may not only control normal angiogenesis, but when administered in sufficient quantities, may provide a naturally-occurring therapeutic inhibitor of diseases associated with angiogenesis.\n\nRecombinant Human PEX (C-Terminal Fragment of Human Matrix Metalloproteinase-2, MMP-2) which comprises C-terminal hemopexin-like domain (amino acids 445-635), Recombinant Human PEX is a noncatalytic Metalloproteinase Fragment with Integrin Binding Activity and can inhibit cell associated collagenolytic activity both in vitro and in vivo. PEX is expressed as inclusion body in E. coli and subsequently refolded in vitro to get biological activity.\n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser.\n\nBiological Activity:\nThe bioactivity was measured by HMEC cell line, PEX can inhibit the transmembrane activity of HMEC under the stimulation of VEGF.\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm. \n2. Analysis by RP-HPLC, using a calibrated solution of PEX as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

M2420-55A

Size

2ug

Form

Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with sterile dH2O to a concentration > 100ug/ml.

Purity

Chromatographically purified ((same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain). \nDimers and Aggregates: < 1% as determined by silver-stained SDS-PAGE gel analysis.\nEndotoxin: < 0.1ng/ug (IEU/ug) of PEX.

References

1