Matrix Metalloproteinase 3 Activity Kit, BioAssay(TM) (MMP3, MMP-3, Stromelysin-1, SL-1, STMY1, Transin-1)

The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E- cadherin, a known contributor to cancer development. In this MMP-3 Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Em/Ex = 325/393 nm.\n\nSample Type:\nCell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food etc. \n\nIntended Use:\nThe kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity.\n\nSensitivity:\n(same/less than)50uU\n\nSpecificity:\nMammalian\n\nKit Components:\nMMP-3 Assay Buffer: 1x25ml\nMMP-3 Substrate: 1x200ul \nMca Standard (1mM): 1x20ul\nMMP-3 Positive Control: 1x1 vial\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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