Matrix Metalloproteinase 3 Inhibitor Screening Kit, BioAssay(TM) (MMP3, MMP-3, Stromelysin-1, SL-1, STMY1, Transin-1)

The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.\n\nSample Type:\nTest inhibitors for MMP-3 \n\nIntended Use:\nThe kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of MMP-3 inhibition. The kit can be used to screen inhibitors of MMP-3.\n\nTest Principle:\nMMP-3 Inhibitor Screening Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Em/Ex = 325/393nm.\n\nKit Components:\nMMP-3 Assay Buffer: 1x25 ml\nMMP-3 Substrate: 1x200ul\nMMP-3 Enzyme (lyophilized): 1x1 vial\nInhibitor Control (0.1mM GM6001): 1x20ul\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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