Matrix Metalloproteinase 7, Recombinant Human (MMP-7, Matrilysin, Pump1, Uterine Metalloproteinase)

Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28kD proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18kD active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.\n\nRecombinant Matrix Metalloproteinase-7 is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 19.13kD.\n\nBiological Activity:\nThe specific activity was found to be 3100 IU/mg.\n\nUnit Definition:\nOne unit is defined as the digestion of 1ug Azocoll/min at 37 degrees C.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilutions: \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2423-20A

Size

5ug

Form

Supplied as a liquid in 10mM HEPES, pH 7.4, 5mM calcium chloride, 150mM sodium chloride. Endotoxin:\n(same/less than) 0.1ng/ug (IEU/ug)

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Inhibitory effects of lignans on the activity of human matrix metalloproteinase 7 (matrilysin). Muta Y, Oyama S, Shimada M, J Agric Food Chem 2004 Sep 22;52(19):5888-94 2. Increased expression of matrix metalloproteinase-7 in invasive early gastric cancer. Kitoh T, Yanai H, Nakamura Y, Okita K, J Gastroenterol 2004;39(5):434-40 3. Correlation of lymph node metastases and expression of matrix metalloproteinase-7 in patients with gastric cancer. Ajisaka H, Yonemura Y, Hepatogastroenterology 2004 May-Jun;51(57):900-5 4. Epidermal growth factor upregulates matrix metalloproteinase-7 expression through activation of PEA3 transcription factors. Lynch CC, Crawford HC, McDonnell S, Int J Oncol 2004 Jun;24(6):1565-72 5. Matrilysin (matrix metalloproteinase-7): a new promising drug target in cancer and inflammation? Wielockx B, Libert C, Cytokine Growth Factor Rev 2004 Apr-Jun;15(2-3):111-5 6. Comprehensive analysis of matrix metalloproteinase and tissue inhibitor expression in pancreatic cancer: increased expression of matrix metalloproteinase-7 predicts poor survival. Jones LE, Humphreys MJ, Neoptolemos JP, Clin Cancer Res 2004 Apr 15;10(8):2832-45.