MBL-C (Mannose-binding Protein C, Mannan-binding Protein, RA-reactive Factor P28A Subunit, RARF/P28A, Mbl2)

Mannose binding lectin (MBL), also called mannose- or mannan-binding protein (MBP), is a member of the group of collectins. MBL is an important pattern-recognition receptor in the innate immune system. The protein mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. Binding of MBL leads to the activation of MBL- associated serine proteases (MASP's). Activated MASP-2 cleaves C4 and C2 in a similar way as C1s do for the classical pathway (CP) leading to the formation of C4b2a, cleavage of the classical pathway convertase C3, and eventually complement activation up to the formation of the membrane attack complex. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. MBL is predominantly synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. This monoclonal antibody is a calcium-dependent antibody.\n\nApplications:\nSuitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1ug/ml TBS/Tween-20/Ca\nImmunofluorescence: Hyphae of C. albicans were fixed on poly-l-lysin coated slides with ice-cold aceton. Slides were incubated for 1h at RT\nImmunohistochemistry (frozen): (7um) of mouse tissue were acetone fixed and stained o/n at 4 degrees C in TBS/2% goat serum\nWestern Blot: On a 12% non-reducing SDS-PAGE a prominent band of ca. 50kD is detected. Under reducing conditions a 26kD band is detected\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2250-01H

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Liu, H et al; Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. Scand J Immunol 2001, 53: 489. 2. Phaneuf, L et al; Binding of mouse mannan-binding lectin to different bacterial pathogens of mice. Vet Immunol Immunopathol 2007, 118: 129. 3. Held, K et al; Increased susceptibility of complement factor B/C2 double knockout mice and mannan binding lectin knockout mice to systemic infection with Candida albicans. Mol Immunol 2008, 45: 3934. 4. Matthijsen, R et al; Macrophage-specific expression of mannose-binding lectin controls atherosclerosis in low-density lipoprotein receptor deficient mice. Circulation 2009, 119: 2188. 5. Pumidonming, W et al, Binding to complement factors and activation of the alternative pathway by Acanthamoeba. Immunobiol. 2010, 216: 225.

Additional Info

Recognizes mouse MBL-C.