MCP3, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 3, MARC)

Recombinant Human MCP-3 is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 9011 Dalton.\n\nAA Sequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Val-Gly-Ile.\n\nBiological Activity:\nMCP-3 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-3 to chemoattract human peripheral blood at 10-100ng/ml.\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2750-17P

Size

2ug

Form

Supplied as a lyophilized powder. No additives. Reconstitute with sterile dH2O, 0.1% HSA or BSA to > 0.1mg/ml.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: (same/less than)0.1ng/ug (IEU/ug) of recombinant Monocyte Chemotactic Protein-3

References

1. MCP-1, MCP-2 and MCP-3 expression in multiple sclerosis lesions: an immunohistochemical and in situ hybridization study. McManus C, et al., J Neuroimmunol 1998 Jun 1;86(1):20-9 2. Monocyte chemotactic protein 4 (MCP-4), a novel structural and functional analogue of MCP-3 and eotaxin. Uguccioni M, et al., J Exp Med 1996 May 1;183(5):2379-84 3. Chemical synthesis, purification and folding of the human monocyte chemotactic proteins MCP-2 and MCP-3 into biologically active chemokines. Proost P, et al., 1995 Feb;7(2):97-104 4. Actions of the chemotactic cytokines MCP-1, MCP-2, MCP-3, RANTES, MIP-1 alpha and MIP-1 beta on human monocytes. Uguccioni M, et al., Eur J Immunol 1995 Jan;25(1):64-8 5. Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines. Opdenakker G, Froyen G, Proost P, Biochem Biophys Res Commun 1993 Mar 15;191(2):535-42 6. Minty A, et al., Eur Cytokine Netw 1993 Mar-Apr;4(2):99-110.