MCP4, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 4)

Recombinant Human MCP-4 is a non-glycosylated, Polypeptide chain containing 74 amino acids and having a molecular mass of 8510 Dalton.\n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Leu.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity:\nMCP-4 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-4 to chemoattaract human monocytes at 10-100ng/ml.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of Human Monocyte Chemotactic Protein-4.\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm. \n2. Analysis by RP-HPLC, using a calibrated solution of Human MCP-4 as a Reference Standard.\n\nReconstitution:\nReconstitute the lyophilized MCP-4 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2750-18B1

Size

2ug

Form

Supplied as a lyophilized powder. No additives.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Monocyte chemotactic protein-4 (MCP-4; CCL-13): a biomarker of asthma. Kalayci O, Sonna LA, Camargo CA Jr, J Asthma 2004 Feb;41(1):27-33 2. Th2- and to a lesser extent Th1-type cytokines upregulate the production of both CXC (IL-8 and gro-alpha) and CC (RANTES, eotaxin, eotaxin-2, MCP-3 and MCP-4) chemokines in human airway epithelial cells. Meyer-Hoffert U, Lezcano-Meza D, Montes-Vizuet AR, Int Arch Allergy Immunol 2003 Aug;131(4):264-71 3. IL-4 differentially regulates eotaxin and MCP-4 in lung epithelium and circulating mononuclear cells. Nakamura H, Luster AD, Jedrzkiewicz S, Yamaguchi K, Am J Physiol Lung Cell Mol Physiol 2001 Nov;281(5):L1288-302 4. Potential role for monocyte chemotactic protein-4 (MCP-4) in monocyte/macrophage recruitment in acute renal inflammation. Chakravorty SJ, Howie AJ, Gentle D, J Pathol 2001 Jun;194(2):239-46 5. Cloning of equine chemokines eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4 in dermal fibroblasts. Benarafa C, Cunningham FM, Horohov DW, Vet Immunol Immunopathol 2000 Oct 31;76(3-4):283-98 6. Monocyte chemoattractant protein (MCP)-4 expression in the airways of patients with asthma. Induction in epithelial cells and mononuclear cells by proinflammatory cytokines. Lamkhioued B, Garcia-Zepeda EA, Nakamura H, Allakhverdi Z, Am J Respir Crit Care Med 2000 Aug;162(2 Pt 1):723-32.