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MECR, aa54-373, Recombinant, Human (Mitochondrial Trans-2-enoyl-CoA Reductase, CGI-63, FASN2B, HsNrbf-1, NBRF1, NRBF1, NRBF-1, Nuclear Receptor-binding Factor 1, Trans-2-enoyl-CoA Reductase, Mitochondrial)

Cat no: M2762-50

MECR, aa54-373, Recombinant, Human (Mitochondrial Trans-2-enoyl-CoA Reductase, CGI-63, FASN2B, HsNrbf-1, NBRF1, NRBF1, NRBF-1, Nuclear Receptor-binding Factor 1, Trans-2-enoyl-CoA Reductase, Mitochondrial)

MECR (Mitochondrial trans-2-enoyl-CoA reductase), also known as NRBF1, catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH dependent manner with preference to medium chain length substrate. This protein has a role in the mitochondrial synthesis of fatty acids.\n\nSource:\nRecombinant corresponding to aa54-373 of human MERC 6x his tagged, expressed in E.coli. \n\nAA Sequences: \nMGSSHHHHHH SSGLVPRGSH MPAKVVELKN LELAAVRGSD VRVKMLAAPI NPSDINMIQG NYGLLPELPA VGGNEGVAQV VAVGSNVTGL KPGDWVIPAN AGLGTWRTEA VFSEEALIQV PSDIPLQSAA TLGVNPCTAY RMLMDFEQLQ PGDSVIQNAS NSGVGQAVIQ IAAALGLRTI NVVRDRPDIQ KLSDRLKSLG AEHVITEEEL RRPEMKNFFK DMPQPRLALN CVGGKSSTEL LRQLARGGTM VTYGGMAKQP VVASVSLLIF KDLKLRGFWL SQWKKDHSPD QFKELILTLC DLIRRGQLTA PACSQVPLQD YQSALEASMK PFISSKQILT M\n\nMolecular Weight: 36.9kD (341aa) confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2762-50

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 5mM DTT, 20% glycerol.

Purity

~90% (SDS-PAGE).

References

1. Scicluna EA., et al. (2010) Eur J Clin Microbiol Infect Dis. 29(2):163-70. 2. Chen ZJ., et al. (2008) J Mol Biol. 379(4):830-44.

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