MEK1, MEK2 (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mitogen-activated Protein Kinase Kinase 2, M

MEK1 and MEK2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK1/2 is a critical step in the MAP kinase cascade.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2865-02A1

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Dro/Arth

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Adams, J.P. and Sweatt, J.D., Annu. Rev. Pharmacol. Toxicol. 42 (2002) 135-163. 2. Ahn, N.G. Mol. Cell Biochem. 127-128 (1993) 201 - 209. 3. Crews, C.M., Alessandrini, A. and Erikson, R.L., Science 258 (1992) 478-480. 4. N.G. Ahn, N.G., Robbins, D.G., Haycock, J.W., Seger, R., Cobb, M.H. and Krebs, E.G., J. Neurochem. 59 (1992) 147-156.

Additional Info

Recognizes human MEK1, MEK2. Species Crossreactivity: rat, mouse, drosophila, canine, bovine, chimpanzee, chicken.

Alternative Names

EC=2.7.12.2, Protein Kinase Mitogen Activated Kinase 1, Protein Kinase Mitogen Activated Kinase 2