Metal content analysis is a technique for detecting and quantifying concentration of metal elements in samples, crucial for ensuring the efficacy and safety of bioproducts during their development and manufacturing. Common methods include atomic absorption spectroscopy (AAS), X-ray fluorescence (XRF), and inductively coupled plasma mass spectrometry (ICP-MS). AAS, a straightforward and cost-effective method, analyzes metal content by combusting the sample and measuring the absorbance of specific elements