Metrnl, Recombinant, Mouse (Meteorin-like Protein)

Meteorin-like protein (METRNL) is a 311aa protein that shows 45% homology with Meteorin (in mouse). Meteorin itself is a secreted 30kD protein that is expressed in glial and neuronal cells and their precursors during development and in the adult. In the adult Meteorin is primarily expressed by astrocytes. Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury. It also inhibits sprouting angiogenesis and promotes blood vessel maturation. Mouse Meteorin-like protein is synthesized with a 45aa signal peptide and is selectively expressed in the otic vesicle during inner ear development. Mature mouse Meteorin-like protein shares 79% and 98% aa sequence identity with human and rat Meteorin-like protein, respectively. Alternate splicing generates an additional isoform that lacks the N-terminal 82aa including the signal peptide. Meteorin-like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region. METRNL is the only gene found within the terminus of the human chromosome 17 q-arm which can be deleted in the rare Ring 17 syndrome.\n\nRecombinant protein corresponding to aa46-311 from mouse METRNL expressed in CHO cells (Q8VE43).\n\nSource:\nCHO cells\n\nBiological Activity:\nMeasured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. This protein is able to significantly enhance neurite outgrowth in the range of 60ng/3ul drop - 300ng/3ul drop.\n \nEndotoxin: (same/less than)1EU/1ug (LAL)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M3009-64

Size

25ug

Form

Supplied as a lyophilized powder from PBS. BSA free. Reconstitute with sterile PBS to a concentration of 0.5mg/ml.

Purity

~90% (SDS-PAGE)

References

1. Park, J.A. et al. (2008) Glia 56:247. 2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104. 3. Lee, H.S. et al. (2010) J. Cell Sci.123:1959. 4. Nishino, J. et al. (2004) EMBO J. 23:1998. 5. Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160. 6. Accession # Q8VE43. 7. Ramialison, M. et al. (2008) Genome Biol. 9:R145. 8. Surace, C. et al. (2009) Clin. Genet. 76:256.